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Exosome 核酸蛋白抽提

Exosomal RNA and Protein Extraction kit

Exosome RNA和蛋白提取试剂盒

 

Name

Exosomal RNA and Protein Extraction Kit

Cat. #

P200

Application

For extracting both exosomal RNA and/or exosomal protein from pure exosome isolated by our Exosome Isolation Kits (Cat.#: P100, P101, P120, P121). This product is for research use only.

Product Size

20 reactions

Yield

In each reaction, from 50 µL ~ 100 µL pure exosome, 50 ~ 100 ng exosomal RNA or 150 ~ 200 µg exosomal protein can be extracted.

Shipping / Storage

Ship at 4℃, and store at -20℃ and room temperature (see component table below). Upon receiving keep all bottles upright, at proper temperature (component table below) in dark place.

Shelf Life

 

6 months

Manual (protocol)

  

Component

Component

Amount

Storage

Exosomal Protein Lysis buffer *

2 mL

-20℃

N1

5 mL

Room temperature

N2

1 mL

Room temperature

N3

2.5 mL

Room temperature

N4

10 mL

Room temperature

RNA Elution buffer

0.5 mL

Room temperature

 

* Store at 4℃ for 7 days or aliquot and store at -20℃ for up to 3 months.

Remarks

Important: RNA is sensitive to RNase. Before starting RNA extraction, prepare clean lab bench and wipe working surface and pipettors with RNase decontamination solution, such as Ambion® RNaseZap®. Always wear clean laboratory gloves during

 

 

 

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