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Exosome 转染

ExoFectin® sRNA-into-Exosome Kit (Electro)

转染试剂盒,将外源miRNA and siRNA 导入Exosome!

 

 

 

 

Cat. #

P400

 

Application

Load nuclei acids ( miRNA and siRNA ) into pure exosomes ( isolated by our kits: Cat.# P100, P101, P120, P121 ). This product is for research use only.

Product Size

10 reactions

Yield

50% ~ 85% transfection efficiency

Description

ExoFectin® sRNA-into-Exosome Kit is a proprietary formation designed for the delivery of small RNA including miRNA and siRNA into exosome. This kit provides the following advantages:

Shipping / Storage

Ship and store at 4℃. (Do not freeze.)

Shelf Life

6 months

Manual (protocol)

 

Component

Components

Amount

Storage

ExoFectin Solution A

700 ul

4℃

ExoFectin Solution B

400 ul

4℃

Electroporation cuvettes

10

Room Temperature

Sterile transfer pipettes

10

Room Temperature

Remarks

The efficiency of electroporation transfection depends on the quality of the exosomes. We do not suggest to start with impure exosome samples prepared by PEG method.

The efficiency of electroporation also depends on the optimal concentration of the exosomes and small RNA. Different RNA and exosome from different source may have different optimal concentration. We suggest customer to test different combinations in the first experiment.

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