载体名称 |
启动子 |
RNA启动子 |
报告基因 |
其他重要特征 |
cDNA过表达 |
shRNA |
MiRNA过表达 |
MiRNA抑制 |
pCDH-CMV-MCS-EF1-Puro |
CMV、EF1 |
— |
Puro |
MCS位点、SV40多聚A加尾信号 |
● |
|
● |
|
pCDH-CMV-MCS-EF1-copGFP |
CMV、EF1 |
— |
GFP |
MCS位点、SV40多聚A加尾信号 |
● |
|
● |
|
pCDH-CMV-MCS-EF1-RFP |
CMV、EF1 |
— |
RFP |
MCS位点、SV40多聚A加尾信号 |
● |
|
● |
|
pCDH-CMV-MCS-EF1-GreenPuro |
CMV、EF1 |
— |
GFP+Puro |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-CMV-MCS-EF1-Neo |
CMV、EF1 |
— |
Neo |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-CMV-MCS-EF1-Hygro |
CMV、EF1 |
— |
Hygro |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-UbC-MCS-EF1-Hygro |
UbC、EF1 |
— |
Hygro |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-MSCV-MCS-EF1-Puro |
MSCV、EF1 |
— |
Puro |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-MSCV-MCS-EF1-GFP |
MSCV、EF1 |
— |
GFP |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-MSCV-MCS-EF1-GFP+Puro |
MSCV、EF1 |
— |
GFP+Puro |
MCS位点、SV40多聚A加尾信号 |
● |
|
|
|
pCDH-EF1-MCS-T2A-Puro |
EF1-α |
— |
Puro |
T2A肽链 |
● |
|
|
|
pCDH-EF1-MCS-T2A-copGFP |
EF1-α |
— |
GFP |
T2A肽链 |
● |
|
|
|
pCDH-EF1-MCS-IRES-GFP |
EF1-α |
— |
GFP |
IRES双顺反子 |
● |
|
|
|
pCDH-EF1-MCS-IRES-RFP |
EF1-α |
— |
RFP |
IRES双顺反子 |
● |
|
|
|
pCDH-EF1-MCS-IRES-Puro |
EF1-α |
— |
Puro |
IRES双顺反子 |
● |
|
|
|
pCDH-EF1-MCS-IRES-Neo |
EF1-α |
— |
Neo |
IRES双顺反子 |
● |
|
|
|
pCDH-UbC-MCS-IRES-GFP |
UbC |
— |
GFP |
IRES双顺反子 |
● |
|
|
|
pCDH-MCS-T2A-Puro-MSCV |
MSCV |
— |
Puro |
T2A肽链 |
● |
|
|
|
pCDH-MCS-T2A-copGFP-MSCV |
MSCV |
— |
GFP |
T2A肽链 |
● |
|
|
|
pCDF1-CMV-MCS1 (FIV来源) |
CMV |
— |
- |
CMV/5’LTR |
● |
|
|
|
pCDF 1-CMV-MCS2-EF1-Puro (FIV来源) |
CMV、EF1 |
— |
Puro |
CMV/5’LTR |
● |
|
|
|
pCDF 1-CMV-MCS2-EF1-copGFP (FIV来源) |
CMV、EF1 |
— |
GFP |
CMV/5’LTR |
● |
|
|
|
pPS-EF1-LCS-T2A-RFP |
EF1-α |
— |
RFP |
LCS位点、T2A肽链、HA标签、 Kozak序列 |
● |
|
|
|
pPS-EF1-LCS-T2A-GFP |
EF1-α |
— |
GFP |
LCS位点、T2A肽链、HA标签、 Kozak序列 |
● |
|
|
|
pPS-EF1-LCS-T2A-Puro |
EF1-α |
— |
Puro |
LCS位点、T2A肽链、HA标签、 Kozak序列 |
● |
|
|
|
pSIH1-CMV-Puro-H1-MCS |
CMV |
H1 |
Puro |
杂交型RSV/5’LTR |
|
● |
|
|
pSIH1-CMV-copGFP-H1-MCS |
CMV |
H1 |
GFP |
杂交型RSV/5’LTR |
|
● |
|
|
pSIH1-CMV-H2Kk-H1-MCS |
CMV |
H1 |
H2Kk |
杂交型RSV/5’LTR |
|
● |
|
|
pSIF1-CMV-Puro-H1-MCS(FIV来源) |
CMV |
H1 |
Puro |
杂交型RSV/5’LTR |
|
● |
|
|
pSIF1-CMV-copGFP-H1-MCS(FIV来源) |
CMV |
H1 |
GFP |
杂交型RSV/5’LTR |
|
● |
|
|
pGreenPuro-CMV-GFP-T2A-Puro-H1-MCS |
CMV |
H1 |
Puro和GFP |
T2A肽链、杂交型RSV/5’LTR |
|
● |
|
● |
pGreenPuro-EF1-GFP-T2A-Puro-H1-MCS |
EF1 |
H1 |
Puro和GFP |
T2A肽链、杂交型RSV/5’LTR |
|
● |
|
|
pFIV-CMV-Puro-H1-MCS-U6(FIV来源) |
CMV |
U6正向、 H1反向 |
Puro |
直接转录出双链siRNA |
|
● |
|
|
pFIV-CMV-copGFP-H1-MCS-U6(FIV来源) |
EF1 |
U6正向、 H1反向 |
GFP |
直接转录出双链siRNA |
|
● |
|
|
pPS-CMV-copGFP-H1-LCS |
CMV |
H1 |
GFP |
LCS位点 |
|
● |
|
|
pPS-CMV-Puro-H1-LCS |
CMV |
H1 |
Puro |
LCS位点 |
|
● |
|
|
pPS-CMV-aRFP-H1-LCS |
CMV |
H1 |
RFP |
,
LCS位点 |
|
● |
|
|
pPS-EF1-aRFP-H1-LCS |
EF1 |
H1 |
RFP |
LCS位点 |
|
● |
|
|
pCDF1- CMV-copGFP-Zeo-MCS(FIV来源) |
CMV |
— |
GFP和Zeo |
CMV/5’LTR |
|
|
● |
|
pPS-EF1-copGFP-LCS |
EF1-α |
— |
GFP |
LCS位点 |
|
|
● |
|
pPS-EF1-RFP-LCS |
EF1-α |
— |
RFP |
LCS位点 |
|
|
● |